Construction of an infectious HIV type 1 molecular clone from an African patient with a subtype D/C Recombinant Virus.

نویسندگان

  • Binshan Shi
  • Sean M Philpott
  • Barbara Weiser
  • Carla Kuiken
  • Cheryl Brunner
  • Guowei Fang
  • Keith R Fowke
  • Francis A Plummer
  • Sarah Rowland-Jones
  • Job Bwayo
  • Aggrey O Anzala
  • Joshua Kimani
  • Rupert Kaul
  • Harold Burger
چکیده

The majority of HIV-1 infections worldwide occur in Africa, where subtype B viruses are rare and intersubtype recombinants are common. Pathogenesis and vaccine studies need to focus on viruses derived from African patients, and infectious HIV-1 molecular clones can be useful tools. To clone non-B subtypes and recombinant viruses from patients, we cultivated HIV-1 from the plasma of a Kenyan long-term survivor. Viral DNA was cloned into a plasmid, which was transfected into COS cells; progeny virus was propagated in PBMCs. Sequence analyses revealed that both the patient's plasma HIV-1 RNA and the cloned DNA genomes were recombinants between subtypes D and C; subtype C sequences comprised the nef and LTR regions. The cloned virus used the CCR5 coreceptor and did not form syncytia in vitro. This infectious HIV-1 subtype D/C recombinant molecular clone obtained from a Kenyan long-term survivor promises to be useful to study pathogenesis and vaccine design.

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عنوان ژورنال:
  • AIDS research and human retroviruses

دوره 20 9  شماره 

صفحات  -

تاریخ انتشار 2004